Efficient condensed-phase production of perdeuterated soluble and membrane proteins
نویسندگان
چکیده
منابع مشابه
Determination of the Phase Diagram for Soluble and Membrane Proteins
Methods to efficiently determine the phase behavior of novel proteins have the potential to significantly benefit structural biology efforts. Here, we present protocols to determine both the solubility boundary and the supersolubility boundary for protein/precipitant systems using an evaporation-based crystallization platform. This strategy takes advantage of the well-defined rates of evaporati...
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Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris...
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1. The interactions of four proteins (albumin, myelin basic protein, melittin and glycophorin) with eight neutral or acidic glycosphingolipids, including sulphatides and gangliosides, five zwitterionic or anionic phospholipids and some of their mixtures, were studied in lipid monolayers at the air/145 mM-NaCl interface. 2. In lipid-free interfaces, the surface pressure and surface potential rea...
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An experiment was conducted to investigate the effect of molasses distillers condensed soluble (MDCS) on growth performance, carcass characteristics and intestinal morphology of broilers. A total of 400 one day-old, unsexed Ross 308 broiler chicks were randomly allotted to 20 cages. The dietary MDCS levels incorporated 0% and 4% in the starter and 0%, 4% and 8% in grower diet, respectively. The...
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Electrophoresis of proteins, especially seed proteins, and karyological studies are useful techniques for determining species and solving the problems of modern taxonomy. The present study was carried out on two species of the Apocynaceae family, Rhazya stricta Decasine and Nerium 01eancder.L. Seed samples of these two genera were taken from the south-east of Iran and SDS-PAGE analysis of th...
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ژورنال
عنوان ژورنال: Journal of Structural and Functional Genomics
سال: 2010
ISSN: 1345-711X,1570-0267
DOI: 10.1007/s10969-010-9083-x